First, a note about reservations: GET ONE! call 055-294-883 as soon as you know when you'll be in Florence. Operators speak English and will get you a 15 minute entry window. Take your confirmations number on the appointed day and first go to the ticket pickup (across the courtyard from the entry line), then get in the reservations only line and you're in.
An amazing look at the greatest Italian painters, going in chronological order from very early Italian to High Renaissance and Baroque. Allow yourself about 2 hours if you want to take your time. We got the audio guide, which I would pass on next time. The info was pretty dry (more info on painting styles, rather than explaining the attributes of a particular work) and it was confusing knowing what you were supposed to be looking at.
The highlight was the Botticelli room which contains his two most famous works "Birth of Venus" and "Primavera."
Admission costs €9.50 (reservation is an additional €3, but worth it!) Audio guide is €4 (and not really worth it).
No photos allowed inside the museum.