A scene in Prague? If you're gay and male, Prague is your oyster . . . I'm not sure where all the dykes go though, as I didn't meet a single lesbian while I was out there!! Maybe it was just the cold that kept them in.
Personally, I was based at the Arco Hotel (Vinohrady), a great place to meet single gay male travellers. If you're staying there, you're bound to find someone to explore the scene with if you're on your own.
Stella Bar (Luzicka 2, pr2) is an underground cellar bar somewhat typical of Prague: small, cosy, open for hours, and full of men!! (Tip: go to the "kitchen" at the far left of the bar for a free "cloakroom"). Like other bars in Prague, you do need to ring the bell to gain entry -- and to be let out again!!
Downtown Cafe (Jungmannovo Namesti 21, off Wenceslas Sq. -- go through passage under the Bata Department Store) does great hot chocolates and baguettes, and has really cute gay guys that work/run the place.
A-Club (Millicova 25, Zizkov). At the time of my visit, this was the only lesbian club in Prague and, after plucking up the courage to venture there on my own, I knocked on the door, only to find it was closed until five days after I left!! Disappointing, but it does appear to be the best dyke club in town - - it has a lesbian festival in April, apparently. The spot does have own website, but all in czech . . . check their entry on gayguide.net
Word of warning, not that I particularly experienced this as a dyke, but according to the men I met out in Prague, it appears that a lot of the male scene is dominated by "escorts". Most of the men I met were American, and most of them had met local Czech boys who were only interested in sex if they were going to get paid. One of the local guys I met told me that being an escort is normal for the local gay guys . . . So if you're male and being paid a lot of attention, check why!!
See Gay Guide.